One-Color DNA Labeling Kits

Description Catalog Number Pack Size Kit Capacity Compatible Applications Ordering*
One-Color DNA Labeling Kit 06370438001 1 Kit 96 Cy3 Labeling Reactions Gene Expression Contact Us to Purchase
06370411001 1 Kit 24 Cy3 Labeling Reactions Contact Us to Purchase
* Availability of products varies from country to country.
Show All Hide All

Applications

NimbleGen One-Color DNA Labeling kits (24 and 96 reactions) are designed for convenient and efficient labeling of cDNA samples prior to hybridization to NimbleGen microarrays for Gene Expression analysis. This kit is based on Cy3-random priming and uses a high concentration of exo- Klenow, lacking both proofreading (3’→ 5’) and nick translation(5’ → 3’) nuclease activities, to incorporate dNTPs while replicating the cDNA.

The new labeling kits offer several content and protocol improvements over the older kit: optimized Cy3- random primers, and a simplified “Stop Solution” (used for terminating the labeling reaction) which is now added in a single step. This optimized and streamlined protocol leads to more robust labeling yields, improved signal to noise ratios (CGH/CNV), and will enable you to achieve a higher-throughput yet economical sample analysis. When used in combination with the NimbleGen Hybridization and Wash Buffer kits, the DNA Labeling kits ensure high signal-to-noise ratios and highly sensitive signals for NimbleGen Gene Expression microarray applications.

96 Reaction Kit (Catalog Number: 06370438001)

One-Color DNA Labeling Kit (96 Cy3 Labeling Reactions)

This kit provides sufficient reagents for 96 Cy3 labeling reactions. For all available NimbleGen Gene Expression microarrays, one (1) labeling reaction with 1μg cDNA starting amount is sufficient per hybridization.

Kit Contents

Component Vial Number Cap Color Quantity
PCR Grade Water 1 Clear 1 x 7.25ml
Random Primer Buffer 2 Clear 1 x 4.25ml
Cy3-Random Nonamers 3 Green 4 x 25 O.D.
Klenow Fragment (3'->5' exo-) 50U/µl 4 Yellow 1 x 200 μl
dNTP Mix (10mM each dNTP) 5 Purple 1 x 1.0ml
Stop Solution 6 Clear 1 x 2.1ml

24 Reaction Kit (Catalog Number: 06370411001)

One-Color DNA Labeling Kit (24 Cy3 labeling reactions)

This kit provides sufficient reagents for 24 Cy3 labeling reactions. For all available NimbleGen Gene Expression microarrays, one (1) labeling reaction with 1μg cDNA starting amount is sufficient per hybridization.

Kit Contents

Component Vial Number Cap Color Quantity
PCR Grade Water 1 Clear 1 x 1.85ml
Random Primer Buffer 2 Blue 1 x 1.1ml
Cy3-Random Nonamers 3 Green 1 x 25 O.D.
Klenow Fragment (3'->5' exo-) 50U/µl 4 Yellow 1 x 50 μl
dNTP Mix (10mM each dNTP) 5 Purple 1 x 250 μl
Stop Solution 6 Brown 1 x 550 μl

Literature

Brochures & Sales Flyers

Technical Notes

Labeling Protocols

The labeling protocol for Gene Expression arrays can be found in the following User's Guide:

For life science research only. Not for use in diagnostic procedures. This website contains information on products which is targeted to a wide range of audiences and could contain product details or information otherwise not accessible or valid in your country. Please be aware that we do not take any responsibility for accessing such information which may not comply with any valid legal process, regulation, registration or usage in the country of your origin.